iolE [2019-08-13 09:25:16]

2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Locus: BSU_39720

Isoelectric point: 5.21

Molecular weight: 33.44 kDa

Protein length: 297 aa

Gene length: 894 bp

Function: myo-inositol catabolism

Product: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Essential: no

E.C.: 4.2.1.44

Synonyms: yxdE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 4,078,173 → 4,079,066

The protein

Catalyzed reaction/ biological activity

• 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H₂O (according to Swiss-Prot)

Protein family

• IolE/MocC family (single member, according to UniProt)

Structure

• 3CNY (from Lactobacillus plantarum wcfs1 mutant, 63% identity, 78% similarity)

Expression and Regulation

Operons

• Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • IolR: repression, PubMed, in IolR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by inositol (IolR) PubMed

Biological materials

Mutant

• MGNA-B774 (iolE::erm), available at the NBRP B. subtilis, Japan
• BKE39720 (ΔiolE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
• BKK39720 (ΔiolE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT, downstream forward: _UP4_CTGGCTTAATGGGGAATGAA

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

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